|Index name:||Human High mobility group protein B1,HMGB-1 elisa kit|
|Inspection range:||6 ng/L -200 ng/L|
|Product Type:|| ELISA kit|
|Quality guarantee period:||for 6 months|
Coated ELISA plate
12-Well * 8 Tubes
Washing concentrate (30X)
Seal plate membrane
Chromogen solution A
Chromogen solution B
INTENDED USEThe kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of this index in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
STORAGEFor unopened kit: All the reagents should be kept according to the labels on vials, Washing concentrate (30X) and the Stop Solution should be stored at 4℃ upon receipt while the others should be at -20℃
PRECISIONIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
1．Store the kit at 4°C upon receipt.The kit should be equilibrat4ed to room temperature before the assay. Remove any unneeded strips from Human MUSK Antibody-Coated plate, reseal them in zip-lock foil and keep at 4°C.
2．Precipitates may appear in concentrated washing buffer. Please heat the buffer to dissolve all the precipitates, which will not affect the results.
3．Accurate pipette should be used to avoid experimental error. Samples should be added to the Microplate in less than 5 minutes. If a large number of samples are included, multiple channel pipette is recommended.
4．Standard curve should be included in every assay. Replicate wells are recommended. If the OD value of the sample is greater than the first well of standards, please dilute the sample (n times) before test. When calculating the original MUSK ?concentration, please multiply the total dilution factor (XnX5).
5．In order to avoid cross-contamination, Microplate sealers are for one-time use only.
6．Please keep Substrate away from light.
7．All the operation should be accordance with the manufacturer's instructions strictly. The results determined by the Microtiter Plate Reader.
8．All the samples, washing buffer and wastes should be treated as infectious agents.
9．Reagents from different lots should not be mixed.